Barnase folding pathway
This diagram colours residues
according to whether they are
folded or unfolded in the transition
Red Pink Magenta Purple Blue
Broken <-----------------------> Formed
Yellow indicates the sites of the engineered disulphide bridges.
The bridge on the right (85 / 102) is in a region which is still
partially formed in the TS, i.e. it breaks late on the unfolding
pathway. The bridge on the left (43 / 80) is in a region that is
completely broken in the TS.
Clarke & Fersht Biochemistry, 32, 4322-9 (1993).