PPT Slide

Detection of DNA hybridization by polarization

modulation IR reflection-absorption spectroscopy

Signature of hybridization

can be observed in the

C=O stretching and NH2

bending region between

1600 and 1750 cm-1

Brewer and Franzen, Langmuir, 2002, 18, 4460

ssDNA ____

dsDNA ____

Hybridize

1x SSC

Heat and anneal

Au surface

Au surface

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